Biophysics and Chemical Physics
Coordinated by: Institute of Physics Charles University
Study branch coordinator: prof. RNDr. Marek Procházka, Ph.D.
Profile of graduates and study aims:
The graduate knows quantum theory and the statistical physics of molecules and molecular systems, experimental methods of biophysics and chemical physics, especially optical and other spectroscopic methods, structural analysis and imaging techniques. Graduates of the theoretical specialization have deeper knowledge in the field of quantum chemistry, molecular dynamics or advanced theoretical spectroscopy. Graduates of the experimental specialization have deeper knowledge in the field of biochemistry and molecular biology, biophysics of photosynthesis or structural methods. Through regular seminars, master's theses, and thematically focused lectures, graduates have gained an idea of problems current in various fields and of methods of scientific work. They are proficient in communicating professional knowledge in the form of presentations and written texts, also in English. Some graduates can expect to pursue a career as a researcher. The acquired education also gives graduates employment opportunities in interdisciplinary teams dealing with physics, biology, chemistry, medicine, materials research, bio- and nano-technologies or pharmacy.
3.1 Recommended Course of Study
The field offers students two specializations - experimental and theoretical. Students usually select a specialization after the end of the first semester (first year of studies, winter semester). Until then, the courses of study in both specializations are the same.
Within each specialization, students have the opportunity to narrow the focus of their studies, which will be reflected in the choice of questions for the final state examination. Students choose two thematic areas (from three possible) and within these courses from the compulsory and optional courses of set I. In the experimental specialization, these include: 1. Biochemistry and molecular biology (courses NBCM012, NBCM008), 2. Optical spectroscopy and biophysics of photosynthesis (courses NBCM179, NBCM088) and 3. Structural methods (courses NBCM098, NBCM112). In the theoretical specialization, these include: 1. Quantum chemistry (courses NBCM121, NBCM122, NBCM155), 2. Molecular dynamics and statistics (courses NBCM346, NBCM100, NFPL004) and 3. Advanced theoretical spectroscopy (courses NBCM154, NBCM027, NOOE119).
Prerequisite for this study programme is a bachelor-level knowledge of quantum theory and general chemistry.
Specialization: Experimental biophysics and chemical physics
Compulsory and elective courses – set I (25 credits)
First year
Code | Subject | Credits | Winter | Summer | |
NBCM010 | Bioorganic chemistry | 4 | 2/1 C+Ex | — | |
NBCM177 | Experimental methods of biophysics and chemical physics I | 6 | 4/0 Ex | — | |
NBCM160 | Classical and quantum statistical physics of molecular systems | 4 | 3/0 Ex | — | |
NBCM039 | Quantum Theory of Molecules | 7 | 3/2 C+Ex | — | |
NBCM095 | Practical Course in Experimental Methods of Biophysics and Chemical Physics I | 7 | 0/5 MC | — | |
NSZZ023 | Diploma Thesis I | 6 | — | 0/4 C | |
NBCM178 | Experimental methods of biophysics and chemical physics II | 3 | — | 2/0 Ex | |
NBCM088 | Biophysics of Photosynthesis | 3 | — | 2/0 Ex | |
NBCM012 | Biochemistry | 4 | — | 3/0 Ex | |
NBCM112 | Magnetic Resonance Methods in Biophysics | 4 | — | 3/0 Ex | |
NBCM179 | Advanced methods of optical spectroscopy | 4 | — | 3/0 Ex | |
NBCM103 | Practical Course in Experimental Methods of Biophysics and Chemical Physics II | 7 | — | 0/5 MC |
Second year
Code | Subject | Credits | Winter | Summer | |
NSZZ024 | Diploma Thesis II | 9 | 0/6 C | — | |
NBCM175 | Seminar of biophysics and chemical physics I | 3 | 0/2 C | — | |
NSZZ025 | Diploma Thesis III | 15 | — | 0/10 C | |
NBCM176 | Seminar of biophysics and chemical physics II | 3 | — | 0/2 C | |
NBCM008 | Molecular and Cell Biology for Biophysicist | 4 | 3/0 Ex | — | |
NBCM098 | X-ray and Electron Structure Analysis of Biomolecules and Macromolecules | 3 | 2/0 Ex | — | |
NBCM165 | Theoretical bases of molecular spectroscopy | 3 | 2/0 Ex | — |
Elective Courses – set II (15 credits)
Code | Subject | Credits | Winter | Summer | |
NBCM101 | Detection and Spectroscopy of Single Molecules | 3 | 2/0 Ex | — | |
NBCM033 | Physical Principles of Photosynthesis | 3 | 2/0 Ex | — | |
NFPL185 | Advanced High Resolution NMR Spectroscopy | 5 | 2/1 C+Ex | — | |
NBCM158 | Practical aspects of experimental data treatment | 3 | 1/1 Ex | — | |
NBCM014 | Structure, Dynamics and Functions of Biomembranes | 3 | 2/0 Ex | — | |
NBCM023 | Importance and Functions of Metal Ions in Biological Systems | 3 | 2/0 Ex | — | |
NBCM102 | Fundamentals of Classical Radiometry and Photometry | 3 | 2/0 Ex | — | |
NBCM026 | Experimental Technology in Molecular Spectroscopy | 3 | — | 2/0 Ex | |
NFPL179 | Quantum Description of NMR | 5 | — | 2/1 C+Ex | |
NBCM114 | Optical Microscopy and Selected Imaging Techniques in Biophysics | 3 | — | 2/0 Ex | |
NOOE012 | Scattering Methods in Optical Spectroscopy | 3 | — | 2/0 Ex | |
NBCM097 | Surface-Enhanced Raman Spectroscopy | 3 | — | 2/0 Ex | |
NBCM172 | Two-dimensional electronic spectroscopy | 3 | 1/1 C+Ex | 1/1 C+Ex | |
NBCM316 | Computer Modelling of Biomolecules | 4 | 1/2 C+Ex | 1/2 C+Ex | |
NBCM018 | One-week Practical Course in Biochemistry | 3 | 0/2 C | 0/2 C |
Recommended optional courses
Code | Subject | Credits | Winter | Summer | |
NBCM121 | Ab Initio Methods and Density Functional Theory I | 5 | — | 2/1 C+Ex | |
NBCM122 | Ab Initio Methods and Density Functional Theory II | 3 | 2/1 C+Ex | — | |
NBCM173 | Ab-initio methods for periodic systems | 3 | 2/0 Ex | — | |
NBCM307 | Astrobiology | 3 | 2/1 Ex | — | |
NBCM024 | Yeast Biology | 3 | — | 2/0 Ex | |
NBCM150 | Physical observation of nano-objects | 5 | 2/1 C+Ex | 2/1 C+Ex | |
NAFY018 | Chemistry for Physicists | 4 | 2/1 C+Ex | — | |
NBCM106 | Chemistry for Physicists II — Analytical Chemistry | 6 | — | 2/2 C+Ex | |
NBCM156 | Chiroptic spectroscopy | 3 | — | 2/0 Ex | |
NBCM154 | Quantum electrodynamics | 3 | — | 2/0 Ex | |
NBCM134 | Quantum Theory of Resonances | 3 | — | 2/0 Ex | |
NBCM051 | Molecular Dynamics and Monte Carlo Methods | 5 | 2/1 C+Ex | — | |
NBCM346 | Molecular dynamics I | 5 | — | 2/1 C+Ex | |
NBCM347 | Molecular dynamics II | 5 | 2/1 C+Ex | — | |
NBCM181 | Molecular dynamics — calculations of free energy | 3 | 1/2 MC | 1/2 MC | |
NBCM055 | Molecular Simulations for solving of material structure | 5 | 2/1 C+Ex | 2/1 C+Ex | |
NBCM149 | Nanotechnology in biology | 3 | 2/0 C | 2/0 C | |
NOOE119 | Nonlinear Optical Spectroscopy | 3 | — | 2/0 Ex | |
NFPL004 | Nonequilibrium Statistical Physics and Thermodynamics | 3 | 2/0 Ex | — | |
NBCM305 | Optical Sensors | 3 | 2/0 Ex | — | |
NBCM099 | Practical Exercises in Quantum Theory of Molecules I | 4 | — | 0/3 C | |
NBCM116 | Practical Exercises in Quantum Theory of Molecules II | 4 | 0/3 C | — | |
NAFY080 | Preparation of Biological Samples | 3 | — | 2/0 Ex | |
NOOE015 | Seminar | 2 | — | 0/1 C | |
NFPL186 | Seminar on High Resolution NMR Spectroscopy | 3 | 0/2 C | 0/2 C | |
NBCM027 | Symmetry of Molecules | 4 | — | 2/1 C+Ex | |
NFPL003 | Synthetic Problems of Quantum Theory | 3 | — | 2/0 C | |
NBCM115 | Scientific Photography and Related Imaging Techniques | 3 | 1/1 Ex | — | |
NPRF005 | UNIX and LINUX for Physicists | 3 | 2/0 C | — | |
NBCM159 | Introduction to Computer Control of Experiment | 4 | — | 1/2 MC | |
NBCM308 | Introduction to Protein Structure Studies | 3 | — | 2/0 Ex | |
NBCM100 | Computational Experiments in Molecular Theory I | 4 | — | 0/3 MC | |
NBCM125 | Computational Experiments in Molecular Theory II | 6 | — | 0/4 MC | |
NBCM041 | Fundamentals of Energy Transfer in Molecular Systems I | 3 | 2/0 Ex | — |
Specialization: Theoretical biophysics and chemical physics
Compulsory and elective courses – set I (25 credits)
First year
Code | Subject | Credits | Winter | Summer | |
NBCM010 | Bioorganic chemistry | 4 | 2/1 C+Ex | — | |
NBCM177 | Experimental methods of biophysics and chemical physics I | 6 | 4/0 Ex | — | |
NBCM160 | Classical and quantum statistical physics of molecular systems | 4 | 3/0 Ex | — | |
NBCM039 | Quantum Theory of Molecules | 7 | 3/2 C+Ex | — | |
NBCM095 | Practical Course in Experimental Methods of Biophysics and Chemical Physics I | 7 | 0/5 MC | — | |
NSZZ023 | Diploma Thesis I | 6 | — | 0/4 C | |
NBCM178 | Experimental methods of biophysics and chemical physics II | 3 | — | 2/0 Ex | |
NBCM121 | Ab Initio Methods and Density Functional Theory I | 5 | — | 2/1 C+Ex | |
NBCM154 | Quantum electrodynamics | 3 | — | 2/0 Ex | |
NBCM346 | Molecular dynamics I | 5 | — | 2/1 C+Ex | |
NBCM100 | Computational Experiments in Molecular Theory I | 4 | — | 0/3 MC |
Second year
Code | Subject | Credits | Winter | Summer | |
NSZZ024 | Diploma Thesis II | 9 | 0/6 C | — | |
NBCM175 | Seminar of biophysics and chemical physics I | 3 | 0/2 C | — | |
NSZZ025 | Diploma Thesis III | 15 | — | 0/10 C | |
NBCM176 | Seminar of biophysics and chemical physics II | 3 | — | 0/2 C | |
NBCM122 | Ab Initio Methods and Density Functional Theory II | 3 | 2/1 C+Ex | — | |
NBCM155 | Field theory methods in the theory of many particles | 3 | 2/0 Ex | — | |
NFPL004 | Nonequilibrium Statistical Physics and Thermodynamics | 3 | 2/0 Ex | — | |
NBCM027 | Symmetry of Molecules | 4 | — | 2/1 C+Ex | |
NBCM165 | Theoretical bases of molecular spectroscopy | 3 | 2/0 Ex | — | |
NOOE119 | Nonlinear Optical Spectroscopy | 3 | — | 2/0 Ex |
Elective Courses – set II (15 credits)
Code | Subject | Credits | Winter | Summer | |
NBCM067 | Quantum Optics I | 5 | 2/1 C+Ex | — | |
NBCM347 | Molecular dynamics II | 5 | 2/1 C+Ex | — | |
NBCM131 | Advanced Methods in Molecular Dynamics | 3 | 2/0 Ex | — | |
NBCM041 | Fundamentals of Energy Transfer in Molecular Systems I | 3 | 2/0 Ex | — | |
NBCM093 | Quantum Optics II | 5 | — | 2/1 C+Ex | |
NBCM134 | Quantum Theory of Resonances | 3 | — | 2/0 Ex | |
NBCM099 | Practical Exercises in Quantum Theory of Molecules I | 4 | — | 0/3 C | |
NBCM116 | Practical Exercises in Quantum Theory of Molecules II | 4 | 0/3 C | — | |
NBCM125 | Computational Experiments in Molecular Theory II | 6 | — | 0/4 MC | |
NBCM055 | Molecular Simulations for solving of material structure | 5 | 2/1 C+Ex | 2/1 C+Ex | |
NBCM180 | Theoretical seminar of biophysics and chemical physics | 2 | 0/1 C | 0/1 C |
Recommended optional courses
Code | Subject | Credits | Winter | Summer | |
NBCM173 | Ab-initio methods for periodic systems | 3 | 2/0 Ex | — | |
NBCM307 | Astrobiology | 3 | 2/1 Ex | — | |
NBCM184 | Asymptotic Methods in Physics | 5 | 2/1 C+Ex | — | |
NBCM088 | Biophysics of Photosynthesis | 3 | — | 2/0 Ex | |
NBCM012 | Biochemistry | 4 | — | 3/0 Ex | |
NBCM101 | Detection and Spectroscopy of Single Molecules | 3 | 2/0 Ex | — | |
NBCM172 | Two-dimensional electronic spectroscopy | 3 | 1/1 C+Ex | 1/1 C+Ex | |
NBCM026 | Experimental Technology in Molecular Spectroscopy | 3 | — | 2/0 Ex | |
NBCM150 | Physical observation of nano-objects | 5 | 2/1 C+Ex | 2/1 C+Ex | |
NBCM033 | Physical Principles of Photosynthesis | 3 | 2/0 Ex | — | |
NBCM156 | Chiroptic spectroscopy | 3 | — | 2/0 Ex | |
NBCM067 | Quantum Optics I | 5 | 2/1 C+Ex | — | |
NFPL179 | Quantum Description of NMR | 5 | — | 2/1 C+Ex | |
NBCM112 | Magnetic Resonance Methods in Biophysics | 4 | — | 3/0 Ex | |
NBCM051 | Molecular Dynamics and Monte Carlo Methods | 5 | 2/1 C+Ex | — | |
NBCM008 | Molecular and Cell Biology for Biophysicist | 4 | 3/0 Ex | — | |
NBCM181 | Molecular dynamics — calculations of free energy | 3 | 1/2 MC | 1/2 MC | |
NBCM114 | Optical Microscopy and Selected Imaging Techniques in Biophysics | 3 | — | 2/0 Ex | |
NBCM316 | Computer Modelling of Biomolecules | 4 | 1/2 C+Ex | 1/2 C+Ex | |
NTMF002 | Advanced Quantum Theory | 6 | 3/1 C+Ex | — | |
NFPL185 | Advanced High Resolution NMR Spectroscopy | 5 | 2/1 C+Ex | — | |
NBCM179 | Advanced methods of optical spectroscopy | 4 | — | 3/0 Ex | |
NBCM158 | Practical aspects of experimental data treatment | 3 | 1/1 Ex | — | |
NBCM103 | Practical Course in Experimental Methods of Biophysics and Chemical Physics II | 7 | — | 0/5 MC | |
NBCM098 | X-ray and Electron Structure Analysis of Biomolecules and Macromolecules | 3 | 2/0 Ex | — | |
NOOE012 | Scattering Methods in Optical Spectroscopy | 3 | — | 2/0 Ex | |
NOOE015 | Seminar | 2 | — | 0/1 C | |
NFPL186 | Seminar on High Resolution NMR Spectroscopy | 3 | 0/2 C | 0/2 C | |
NFPL003 | Synthetic Problems of Quantum Theory | 3 | — | 2/0 C | |
NPRF005 | UNIX and LINUX for Physicists | 3 | 2/0 C | — | |
NBCM159 | Introduction to Computer Control of Experiment | 4 | — | 1/2 MC | |
NBCM308 | Introduction to Protein Structure Studies | 3 | — | 2/0 Ex | |
NBCM115 | Scientific Photography and Related Imaging Techniques | 3 | 1/1 Ex | — | |
NBCM102 | Fundamentals of Classical Radiometry and Photometry | 3 | 2/0 Ex | — | |
NBCM042 | Fundamentals of Energy Transfer in Molecular Systems II | 3 | — | 2/0 Ex |
3.2 State Final Exam
Necesary conditions for taking the state final exam
- – earning at least 120 credits during the course of the study
- – passing all compulsory courses
- – obtaining at least 25 credits for elective courses from the set I
- – obtaining at least 15 credits for elective courses from the set II
- – submission of a completed master’s thesis by the submission deadline
- – passing all compulsory courses
Requirements for the oral part of the state final exam
A Common requirements
1 Quantum theory and statistical physics of molecules and molecular systems (one question in the state exam)
- – Antisymmetry of wave function, exchange interaction.
- – Born - Oppenheimer and adiabatic approximation.
- – Hydrogen molecule. Atomic and molecular orbitals.
- – LCAO method and valence bond method, classification of electron levels, Hückel method.
- – One-particle approximation, Hartree and Hartree - Fock equations, Roothaan equations.
- – Fundamentals of density functional theory, Hohenberg-Kohn theorems.
- – Introduction to methods of configuration interaction, coupled clusters and perturbation theory, basic equations and properties, Brillouin theorem.
- – Pauli and Dirac equations. Spin-orbital and spin-spin interaction.
- – Orbital and spin magnetic moment and their interactions with external fields.
- – Quantization of electromagnetic field, interaction of electromagnetic radiation with molecules. Fermi's golden rule.
- – Absorption, stimulated and spontaneous emission. Dipole approximation, selection rules.
- – Force fields in molecular systems.
- – Standard statistical ensembles and distributions, ergodic theorem.
- – Monte Carlo method.
- – Classical molecular dynamics.
- – Liouville equation.
- – Density matrix. Wigner density.
- – Standard quantum statistical distributions.
- – Evolution of the density matrix (Liouville-von Neumann equation).
- – Quantum master equation, reduced densities.
- – Born - Oppenheimer and adiabatic approximation.
2 Experimental methods of biophysics and chemical physics (one question in the state exam)
- – Sources, detectors and spectrum analyzers in optical spectroscopy.
- – Interaction of optical radiation with an isolated molecule. Selection rules for electronic, vibrational and rotational optical transitions.
- – Methods and applications of electron absorption spectroscopy. Excitation and probing method.
- – Methods and applications of vibrational absorption spectroscopy.
- – Methods of elastic, dynamic and Brillouin scattering and their applications.
- – Raman scattering, measurement methods and their applications.
- – Use of polarized radiation and its analysis in optical spectroscopy. Linear and circular dichroism, emission anisotropy.
- – Principles and basic concepts of luminescence (types of luminescence, Jablonsky diagram, kinetics, quantum yield, lifetimes, Franck-Condon principle).
- – Influence of intermolecular interactions on luminescence parameters (environmental influence, resonant energy transfer, emission quenching).
- – Single-molecular spectroscopy. Influence of interaction with the environment on the shape of the spectral line.
- – Measurement of stationary and time-resolved luminescence.
- – Scattering and diffraction of X-rays, electrons and neutrons.
- – Principles of basic diffraction methods. Symmetry and structure of crystals and their determination from the diffraction pattern.
- – Electron microscopy, atomic force microscopy and scanning tunneling microscopy.
- – Mass spectrometry.
- – Nuclear magnetic resonance (NMR): principle, experimental setup, excitation and signal detection, basic pulse sequence.
- – High resolution NMR of organic substances in liquids: interpretation of spectra.
- – Electron paramagnetic resonance: principle, experimental setup, application.
- – Separation methods (centrifugation, chromatography, electrophoresis).
- – Interaction of optical radiation with an isolated molecule. Selection rules for electronic, vibrational and rotational optical transitions.
B Specialization Experimental biophysics and chemical physics
The third question of the state exam is chosen from two thematic areas, which the student chooses according to his focus.
1 Biochemistry and molecular biology
- – Composition and structure of basic biomolecules (nucleic acids, proteins, carbohydrates).
- – Glycolysis and glycolytic reactions. Anaerobic degradation of sugars. Cori cycle.
- – Aerobic degradation of sugars. Formation of acetylcoenzyme A.
- – The citrate cycle and its amorphous nature. Oxidative phosphorylation.
- – Biological membranes, selective permeability of biological membranes, types of transport through the biological membrane.
- – Structure of bacterial and eukaryotic cells, cell division, cell cycle.
- – DNA arrangement in cells, structure and function of chromosomes, chromatin and nucleosomes, centromere and telomere functions, histones, epigenetic inheritance and prions.
- – Genetic information processing, DNA replication, RNA transcription and modification, RNA world, prokaryotic and eukaryotic translation.
- – Basic principles of gene expression regulation, prokaryotic and eukaryotic transcription initiation regulation, gene silencing.
- – Mutations and mutagenesis, DNA damage and repair of damaged DNA, correction of errors caused by DNA replication.
- – Methods of studying DNA and gene expression, genetic engineering, fluorescent proteins.
- – Glycolysis and glycolytic reactions. Anaerobic degradation of sugars. Cori cycle.
2 Optical spectroscopy and biophysics of photosynthesis
- – Fluorescent labels and probes, fluorescent proteins, protein fluorescence.
- – Nonlinear methods of Raman scattering (HRS, SRS, CARS), Raman optical activity (ROA).
- – Advanced techniques of Raman spectroscopy (SERS, CRM, DCDR).
- – Generation and characterization of femtosecond pulses. Fundamentals of 2DES spectroscopy.
- – Nonlinear optical phenomena and their applications in optical spectroscopy.
- – High spectral resolution methods. Low temperature spectroscopy.
- – Transmission and quenching of excitation in photosynthetic antennas.
- – Charge distribution and transfer in low- and high-potential reaction centers.
- – Electron transfer through the photosynthetic membrane, phosphorylation, comparison with the respiratory membrane.
- – Carbon fixation in photosynthesis.
- – Biophysical methods of investigation and measurement of photosynthesis (variable fluorescence, gasometry, photoacoustic spectroscopy).
- – Nonlinear methods of Raman scattering (HRS, SRS, CARS), Raman optical activity (ROA).
3 Structural methods
- – Temperature oscillations and their influence on diffraction recording. Patterson's function and its use in solving crystal structures.
- – Methods for solving the phase problem of structural analysis.
- – Structural factor and Friedel's law. Preferred orientation of crystallites - texture.
- – Comparison, construction and use of transmission and scanning electron microscopes.
- – Principles of sample preparation for TEM and SEM. Mechanism of image formation in TEM and SEM
- – Electric and magnetic moments of atomic nuclei, energy in electric and magnetic fields, the phenomenon of nuclear magnetic resonance (NMR). Nuclear paramagnetism, relaxation processes.
- – High resolution NMR spectroscopy in liquid and solid phase: spin Hamiltonian, types of interactions and their manifestations in spectra, high resolution methods in solid phase.
- – One- and multidimensional pulse NMR: concept, basic pulse sequences, use of coherent polarization transfer and nuclear Overhauser effect.
- – MR imaging: instrumentation, the principle of achieving spatial resolution, methods of contrast, special applications (angiography, fMRI, MRI spectroscopy).
- – Electron spin (paramagnetic) resonance: continuous and pulse methodology of experiment, spin Hamiltonian, interactions and their manifestations in spectra.
- – Methods for solving the phase problem of structural analysis.
B Specialization Theoretical biophysics and chemical physics
The third question of the state exam is chosen from two thematic areas, which the student chooses according to his focus.
1 Quantum chemistry
- – Comparison of restricted and unrestricted Hartree-Fock equations and their properties.
- – Configuration interaction methods, formulation and characteristics.
- – Application of perturbation theory to the calculation of correlation energy, Møller-Plesset method.
- – Coupled cluster method, excitation operators, equations and basic properties.
- – Conceptual density functional theory - chemical potential, hardness and softness of electron density, Fukui function; time-dependent theory.
- – Weak intermolecular interactions; multipole approximation.
- – Configuration interaction methods, formulation and characteristics.
2 Molecular dynamics and statistics
- – Numerical propagators derived from the Liouville operator.
- – Algorithms for pressure and temperature control. Fixation and restriction of degrees of freedom.
- – Non-equilibrium molecular dynamics.
- – Molecular mechanics, parameterization of force fields.
- – Methods of molecular simulations – accounting for non-binding interactions, analysis of trajectories.
- – Stochastic processes (Langevin dynamics, normal and anomalous diffusion).
- – Stochastic quantum dynamics.
- – Entropy in nonequilibrium processes (Boltzmann H-theorem, Jarzynski and fluctuation theorems).
- – Algorithms for pressure and temperature control. Fixation and restriction of degrees of freedom.
3 Advanced theoretical spectroscopy
- – Symmetry in quantum mechanics (quantum numbers, block diagonalization of Hamiltonian).
- – Symmetry in the spectroscopy of atoms and molecules (selection rules, allowed and forbidden transitions, reduction of symmetry in external electromagnetic fields).
- – Scattering of photons by atomic systems (Rayleigh, Raman, resonant and Thomson scattering).
- – Radiative correction to atomic spectra (Lamb shift, self energy of electron and photon).
- – Absorption line-shape (linear response theory, bath correlation function).
- – Perturbation theory for time-resolved nonlinear spectroscopy (pump probe method, photon echo).
- – Symmetry in the spectroscopy of atoms and molecules (selection rules, allowed and forbidden transitions, reduction of symmetry in external electromagnetic fields).