UV-Vis spectrophotometry

UV-Vis spectrophotometry is an analytical technique that measures how much light (electromagnetic radiation) of a specific wavelength a sample absorbs or transmits. The principle of this method lies in the interaction of light with the electrons in the sample. For quantitative analysis, the Beer-Lambert Law is used, which describes the relationship between absorption, concentration, and sample thickness.

At our department, we use the Hitachi U-2910 UV-Vis spectrophotometer, operating in the range from 190 nm to 1100 nm with a spectral bandpass of 1.5 nm.

Furthermore, we also have an Integrating Sphere, model ISP-50-8-R-GT, from Ocean Optics, available, which allows us to measure the total reflectance of samples in the spectral range of 200 nm to 2500 nm.